Jan 012000
 

Case of the month is our diagnostic challenge. We present cases seen by Cornell University’s Clinical Pathology Laboratory. These cover the gamut of venous blood smears, cytologic specimens and result (CBC, chemistry) interpretation, including those pesky erroneous results (usually due to preanalytical errors). A new case will be presented every month. Test yourself with the questions and photomicrographs and make your own diagnosis! The answer along with explanations and discussion is provided on the following page to see how close you came to the diagnosis.

We also have an index of our previous cases (with answers) if you wish to see a list of all the cases. Please note, that the search tool does not search case of the month (yet!).

Apr 012017
 

Peripheral blood from a cat

Case information

Whole blood from a 3 year old male neutered Himalayan cat was collected into an EDTA tube and submitted to the Animal Health Diagnostic Center at Cornell University for a complete blood count. The submitting hospital provided no clinical history.

Review the pertinent data generated by the automated hematology analyzer (ADVIA 2120) and evaluate the photomicrographs below to answer the following questions:

Complete Blood Count (CBC) – Truncated; Data from analyzer

Analyzer data

Questions:

  1. For which cell line is the analyzer clearly reporting spuriously decreased results?
  2. What is an artifact on a typical serum biochemistry panel that may be associated with this condition (in dogs), and what other type of specimen could you evaluate to confirm the artifact?
  3. How would you classify the large cells in Figures 2-3?

Figure 1. Peripheral blood smear. (Wright's stain, 20x objective)

Figure 1. Peripheral blood smear (Wright’s stain, 20x objective).

Figure 2. Large cell in the peripheral blood. (Wright's stain, 100x)

Figure 2. Peripheral blood smear (Wright’s stain, 100x objective).

Figure 3. Large cell on a peripheral blood smear. (Wright's stain, 100x)

Figure 3. Peripheral blood smear (Wright’s stain, 100x objective).

Answers on next page.

Mar 012017
 

Laboratory data from a vomiting painful cat

Case Information

A 3 year old male neutered domestic short haired cat, presented to Cornell University Hospital for Animals Emergency Service with a 24 hour history of inappetence and acting painful when picked up. The cat had also vomited several times but was drinking water.

On presentation, the cat was agitated, alert, responsive and vital parameters (temperature, pulse and respiration) were within normal limits. The cat was extremely painful on caudal abdominal palpation, but appeared to have a large bladder. A complete blood count (CBC) and serum chemistry was summited for analysis. A urinary catheter was passed with some difficulty but urine was obtained and submitted for urinalysis.

Review the laboratory data provided and answer the following questions (only abnormal and pertinent laboratory data has been included).

Complete Blood Count (CBC)Slide1
Serum Chemistry PanelSlide2
Urinalysis
Slide3

Questions:

  1. How would you interpret the leukogram?
  2. What type of acid-base disturbance(s) can you identify from the serum chemistry panel?
  3. What other diagnostic tests should be performed in this case?

Answers on next page.

Feb 012017
 

Peritoneal fluid from an alpaca

Case information

A 6 year old female alpaca presented to the Cornell University Large Animal Hospital with a history of recumbency, anorexia, and dull mentation. Initial physical examination and diagnostic testing showed the animal was dehydrated and had clinical signs consistent with a proximal intestinal obstruction (decreased fecal output, significantly distended third gastric compartment, empty small intestine, and a shadowing object in the caudal aspect of the duodenal ampulla on abdominal ultrasonography). The hemogram showed a mild lymphopenia of 0.2 thousand/µL (reference interval, 1.1-5.5 thousand/µL), likely due to stress. Pertinent biochemical results are shown on the table below:

Analyte Result RI Units
Sodium 160 149-157 mEq/L
Chloride 106 106-116 mEq/L
Bicarbonate 41 22-34 mEq/L
AST 1062 119-286 U/L
SDH 117 0-7 U/L
GLDH 540 3-19 U/L
GGT 155 8-35 U/L
Bilirubin 0.1 0-0.1 mg/dL

The patient was taken to surgery for duodenal enterotomy and third gastric compartment gastrotomy. Surgery revealed two bezoars, one partially obstructing the distal third gastric compartment and another obstructing the duodenal ampulla. An increased amount of peritoneal fluid was found adjacent to the obstructed duodenum. The bezoars were surgically removed and a sample of peritoneal fluid was collected and submitted for cytologic evaluation.

The submitted peritoneal fluid was yellow to light-red in color and opaque, with total protein by refractometry of 2.7 g/dL. The total nucleated cell count was 6.5 thousand/µL and a red blood cell count was 65.1 thousand/µL. Direct and sediment smears from the submitted fluid were prepared.

Evaluate the representative photomicrographs of the peritoneal fluid shown below, consider the biochemistry results, and answer the following questions:

  1.  What type of metabolic derangement is present?
  2.  Identify and evaluate the cells labeled as “A” and “B” in Figure 2. 
  3. What additional diagnostic tests would you do in this case?

Fig1. Peritoneal fluid from an alpaca (Wright's stain, 20x objective)

Figure 1. Peritoneal fluid from an alpaca (Wright’s stain, 20x objective)

Fig2. Peritoneal fluid from an alpaca (Wright's stain, 50x objective)

Figure 2. Peritoneal fluid from an alpaca (Wright’s stain, 50x objective)

Figure3. Peritoneal fluid from an alpaca (Wright's stain, 100x objective)

Figure 3. Peritoneal fluid from an alpaca (Wright’s stain, 100x objective)

Figure4. Peritoneal fluid from an alpaca (Gram stain, 100x)

Figure 4. Peritoneal fluid from an alpaca (Gram stain, 100x objective)

 Answers on next page

Jan 012017
 

Tracheal wash from a horse

Case information

An 8 year old Morgan gelding presented to the Cornell University Hospital for Animals with a 3 week history of serohemorrhagic skin lesions and a 1 week history of a progressively worsening cough. The horse had also recently lost 100 lb. On physical examination, multifocal oozing lesions were present on the left hip, lower aspects of three limbs and neck. No abnormal lung sounds were detected on auscultation with or without a rebreathing bag. Thoracic ultrasonographic and radiographic examination revealed diffuse lung consolidation and pleuritis, with a mixed pulmonary pattern and coalescing nodules, respectively.  Blood samples were taken for a hemogram plus fibrinogen by heat precipitation and chemistry profile.

Hemogram abnormalities included a mild neutrophilia of 7.3 thou/uL (reference interval: 2.7–6.6 thou/uL) and a mild hyperfibrinogenemia of 400 mg/dL (reference interval, 0-200 mg/dL). There was a mild hypoalbuminemia (2.5 g/dL, reference interval: 3.0–3.7 g/dL) and hyperglycemia (141 mg/dL, 71-113 mg/dL) on the biochemical panel.

An endoscopic tracheal wash and blind bronchoalveolar lavage were performed and submitted for cytologic evaluation.

Evaluate the representative photomicrographs of the direct smear of the tracheal wash and answer the questions posed below:

  1. What cell types are present in the smears (how would you characterize the results)?
  2. What are your differential diagnoses for the findings?

Fig1_Jan2017

Figure 1: Tracheal wash from a horse (10x objective)

Fig2_Jan2017

Figure 2: Tracheal wash from a horse (50x objective)

Answer on next page

Dec 012016
 

Joint aspirate from a tortoise

Case information:

An approximately 18 month old, male Hermann’s tortoise presented for raspy breathing, exuberant, pink tissue over the eyes and being less active than normal. On physical examination the tortoise was small for his age, had increased respiratory rate and effort, was 5% dehydrated and would not open his eyes. While the tortoise was fully ambulatory, his left rear leg was swollen in the tarsal region. Whole body radiographs were taken to better assess the animal’s overall health. No abnormalities were seen in the lungs, but extensive bone loss and soft tissue swelling was noted in the region of the left tarsus. The left tarsal joint was aspirated and thick, gritty material was obtained. A portion of this sample was submitted for culture, while the remainder of the sample was assessed cytologically. Evaluate the photomicrographs of the submitted joint material and consider the following questions:

Questions:

  1. What types of inflammatory cells are present?
  2. What are the structures indicated by the arrows in Figure 3 (also pictured in Figure 1A)?
  3. What is the final diagnosis based on the cytological findings?

Figure 1A. Left tarsus joint aspirate from a tortoise (Wright's stain 500x)

Figure 1A. Left tarsus joint aspirate from a tortoise (Wright’s stain 500x)

Figure 2A. Left tarsus joint aspirate from a tortoise (Wright's stain 500x)

Figure 2A. Left tarsus joint aspirate from a tortoise (Wright’s stain 500x)

Figure 3A. Left tarsus joint aspirate from a tortoise (Wright's stain 1000x)

Figure 3A. Left tarsus joint aspirate from a tortoise (Wright’s stain 1000x)

Answers are on the next page

Nov 012016
 

Case: Biochemical work-up of a vomiting dog

Case information:

A 2 year-old male castrated Cane Corso presented to the Cornell University Hospital for Animals for a two-day history of vomiting, hypoglycemia, and bradycardia. Blood-work performed at the referring veterinarian revealed a hyponatremia and hyperkalemia. No abnormalities were found on abdominal radiographs. Prior to referring the dog to the Cornell Emergency service, the rDVM began treatment with a bolus of 50% dextrose and 0.9% NaCl. The dog had a previous history of hospitalization for sepsis, vomiting, and diarrhea that had occurred one week after vaccination for rabies and distemper. Consequently, all vaccine protocols were discontinued and were therefore not up-to-date.

On presentation, the dog was quiet but alert, and hypothermic at 97.3ºF. The remainder of the physical examination was within normal limits. The dog was started on intravenous fluids supplemented with glucose. Rapid in-house assessment tests, venous blood gas analysis, complete blood count, and a full serum chemistry panel were performed. Fluids, supplemented with dextrose, were continued overnight and a constant rate infusion of hydrocortisone was started. A free catch urine and stool sample were also submitted for evaluation.

Review the laboratory data provided, and answer the following interpretive questions. (Abnormal and pertinent laboratory data have been included. Test results not included were within reference intervals.)

CBC

Chemistry panel

Blood gases

UA

Questions:

  1. Given the dog’s clinical history and presentation, what is an unexpected finding in the hemogram results?
  2. What are the differential diagnoses for hypocholesterolemia and which is the most likely in this case?
  3. Presuming the dog’s azotemia resolves with fluid therapy, how would you explain the reduced concentrating ability of the kidney?

Answers are on the next page

Oct 012016
 

Conjunctival swab from a cat

Case information

A 6 year old male castrated domestic shorthair cat presented to the Cornell University Hospital for Animals (CUHA) emergency service for evaluation of worsening eye pain and inappetance. The cat was previously seen by the ophthalmology service at CUHA one week prior for evaluation of ocular discharge and conjunctival swelling of the left eye. In the latter examination, there was no evidence of penetrating trauma or foreign body. The cat was diagnosed with conjunctivitis and discharged with erythromycin ophthalmic ointment q 8 hours.

Upon presentation to the emergency service, the cat demonstrated blepharospasm in both eyes. No other physical examination abnormalities were noted. A CBC and serum chemistry panel were performed and were largely unremarkable. A swab of the conjunctiva was performed and applied to a slide for cytologic evaluation. Representative images from the Wright’s stained smears are provided.

Evaluate the provided images below and answer the following questions:

  1. What type of inflammation is present? 
  2. Can you identify the cause of the inflammation? 
  3. What additional diagnostic could be performed to confirm the cytologic diagnosis? 

Figure 1: Conjunctival swab from a cat

Figure 1: Conjunctival swab from a cat

Figure 2: Conjunctival swab from a cat

Figure 2: Conjunctival swab from a cat

Figure 3: Conjunctival swab from a cat

Figure 3: Conjunctival swab from a cat

Figure 4: Conjunctival swab from a cat

Figure 4: Conjunctival swab from a cat

Answer on next page

Sep 012016
 

Synovial Fluid from a Foal

Case Information

A 3-week-old thoroughbred filly was presented to the Cornell University Hospital for Animals (CUHA) for acute left hind limb lameness and fever. Two days prior to presentation to CUHA, the filly was found acutely lame with a suspicion of trauma (stepped on by another horse).

On presentation, the filly weighed 88 lb and had a temperature of 102.9ºF. The foal was quiet, alert and responsive, but was non-weight bearing on the left pelvic limb and had a moderately sized warm swelling over the left hip with no signs of external trauma. The rest of the physical examination was within normal limits. Blood was drawn for a complete blood count (CBC), equine IgG and serum amyloid A (SAA) testing.

The CBC revealed a microcytic normochromic anemia (27% Hct, 34-46%) with a leukocytosis of 15.8 x 103/uL (reference interval, 5.2 – 10.1 x 103/uL) consisting of a mature neutrophilia of 13.1 x 103/uL (reference interval, 2.7 – 6.6 x 103/uL). The SAA concentration was higher than the upper detection limit of 2500 ug/mL and the fibrinogen concentration by heat precipitation was 700 mg/dL (reference interval, 100-200 mg/dL). The serum IgG was 671 mg/dL (reference interval in adults, 984 – 1685 mg/dL).

Radiographic examination of the left pelvic limb and an abdominal ultrasonographic examination revealed no abnormalities. An ultrasound-guided fine needle aspirate was performed on the swelling and submitted to Clinical Pathology for a joint fluid analysis. The fluid was medium red, opaque and had decreased viscosity. The nucleated cell count was 51.5 x 103/uL, the RBC count was 441.2 x 103/uL, and the total protein (refractometer) was 5.7 g/dL.

Examine the representative images of the joint fluid that are provided below and answer the following questions:

  1. How would you classify this joint fluid?
  2. Based on the cytological findings and the history of this foal, what are your differential diagnoses?
  3. What other diagnostic test should be performed?

Fig 1_September2016

Figure 1: Joint fluid direct smear (Wright’s stain, 20x objective)

Fig2_September2016

Figure 2: Joint fluid direct smear (Wright’s stain, 100x objective)

Answers on next page

Aug 012016
 

Liver aspirate from a cat

Case information 

An 11-year-old male castrated domestic longhair cat presented to the Cornell University Hospital for Animals (CUHA) Emergency Service for a five day history of lethargy, anorexia, persistent hyperthermia, and increased liver enzymes. The cat was first brought to the primary care veterinarian for a two day history of lethargy and inappetance. At that time, bloodwork revealed hypoproteinemia, hypoalbuminemia, and hypocholesterolemia. Radiographs of the chest and abdomen showed mild hepatomegaly. The cat was treated with subcutaneous fluids, antibiotics, and prednisone. After one week of treatment, no improvement was noted and the cat was brought to CUHA for further evaluation.

On presentation, the cat was bright, alert, and responsive, but was hyperthermic (104.8°F), tachycardic (210 bpm), and mildly dehydrated (5-7%). Physical examination also revealed mild icterus in both sclera and discomfort on cranial abdominal palpation. Blood was drawn for a CBC and chemistry panel. The CBC showed a mild normocytic normochromic nonregenerative anemia (hematocrit: 29%, reference interval [RI]: 31-48%). Results also showed a normal total leukocyte and neutrophil count with a mild left shift (0.2 thou/uL, RI: 0.0-0.1 thou/uL) and mild toxic change. Abnormal liver biochemical results are shown below:

Analyte Result RI Units
ALT 1817 35-176 U/L
AST 2236 15-44 U/L
ALP 26 13-83 U/L
GGT <3 0-2 U/L
Total bilirubin 4.1 0-0.2 mg/dL
Direct bilirubin 2.9 0-0.1 mg/dL
Indirect bilirubin 1.2 0-0.2 mg/dL

 

 

An abdominal ultrasound examination revealed mild hepatomegaly, with rounding of the edges of the liver, and enlarged jejunal lymph nodes. Ultrasound-guided needle aspirates were taken from each site and samples were submitted for cytologic evaluation.

Examine the representative images of the liver aspirate that are provided below and answer the following questions:

  1. How would you interpret the biochemical results reported above?
  2. Based on the cytologic findings, what are your differential diagnoses for the underlying cause?
  3. What other diagnostic tests could be done in this case? 

Fig1_August2016

Figure 1: Liver aspirate from a cat

Fig2_August2016

Figure 2: Liver aspirate from a cat

Fig3_August2016

Figure 3: Liver aspirate from a cat

Answers on next page

Jul 012016
 

Conjunctival swab from a dog

 

Case information

A 3-month-old neutered male Siberian Husky puppy presented to the referring veterinarian with a 5 week history of respiratory symptoms and rhythmic jaw movements.  An in-clinic hemogram revealed the following:

Table 1: Abbreviated hemogram results
Test Results Units Reference interval
HCT 26 % 37-62
MCV 55 fL 62-74
MCHC 38 g/dL 32-38
Retic 0.5 % Not provided
Abs Retic 24 thou/μL 10-110
WBC 2.4 thou/μL 5.1-16.8
Seg Neut 0.8 thou/μL 3.0-11.6
Lymph 1.1 thou/μL 1.1-5.4
Platelets 164 thou/μL 148-484

 

Results for an in-house biochemistry panel were within provided reference intervals.

The veterinarian treated the puppy for a presumptive pneumonia with antibiotics, but there was little improvement. The dog was subsequently admitted to an emergency clinic for anorexia and dyspnea. When examined at the emergency clinic, the puppy was dyspneic (this was oxygen dependent), with a marginal increase in temperature (102.6ºF), and had a bilateral serous nasal discharge and chemosis. A swab of the dog’s conjunctival mucosa was taken and rolled onto a microscope slide, followed by rapid staining (Diff-quik®).

Evaluate the representative photomicrograph of the conjunctival swab and answer the questions posed below:

  1. Are any abnormalities evident in the conjunctival swab?
  2. Do the observed findings explain the hemogram results?
  3. What are potential explanations for the dog’s microcytosis? 

Fig1_July2016

Figure 1. Conjunctival swab

Fig2_July2016

Figure 2. Conjunctival swab

Answer on next page

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